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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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A method for the production of highly specific polyclonal antibodies

M Diano1, A Le Bivic, M Hirn

  • 1Biologie de la Différenciation Cellulaire, CNRS, Faculté des Sciences de Luminy, Marseille, France.

Analytical Biochemistry
|October 1, 1987
PubMed

Two polyclonal antibodies directed against paramyosin and tropomyosin from Owenia fusiformis (a marine polychete annelid) were obtained using a new method of immunization. After purification by two-dimensional gel electrophoresis, proteins were transferred onto a nitrocellulose sheet using the Western blot technique. The proteins bound to their cellulose support were injected into rabbits without Freund's adjuvant and without solubilization of nitrocellulose with dimethyl sulfoxide. Highly specific polyclonal antibodies were generated.

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