Т₁  mapping of rat lungs in ¹⁹ F MRI using octafluorocyclobutane

To demonstrate the feasibility of using octafluorocyclobutane (OFCB, c-C₄ F₈ ) for T₁ mapping of lungs in ¹⁹ F MRI.

The study was performed at 7 T in three healthy rats and three rats with pulmonary hypertension. To increase the sensitivity of ¹⁹ F MRI, a bent-shaped RF coil with periodic metal strips structure was used. The double flip angle method was used to calculate normalized transmitting RF field (B_1n ⁺ ) maps and for correcting T₁ maps built with the variable flip angle (VFA) method. The ultrashort TE pulse sequence was applied for acquiring MR images throughout the study.

The dependencies of OFCB relaxation times on its partial pressure in mixtures with oxygen, air, helium, and argon were obtained. T₁ of OFCB linearly depended on its partial pressure with the slope of about 0.35 ms/kPa in the case of free diffusion. RF field inhomogeneity leads to distortion of T₁ maps built with the VFA method, and therefore to high standard deviation of T₁ in these maps. To improve the accuracy of the T₁ maps, the B_1n ⁺ maps were applied for VFA correction. This contributed to a 2-3-fold decrease in the SD of T₁ values in the corresponding maps compared with T₁ maps calculated without the correction. Three-dimensional T₁ maps were obtained, and the mean T₁ in healthy rat lungs was 35 ± 10 ms, and in rat lungs with pulmonary hypertension - 41 ± 9 ms.

OFCB has a spin-rotational relaxation mechanism and can be used for ¹⁹ F T₁ mapping of lungs. The calculated OFCB maps captured ventilation defects induced by edema.