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Scanless two-photon voltage imaging.

Ruth R Sims¹, Imane Bendifallah¹, Christiane Grimm¹

¹Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France.

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|February 7, 2023

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This summary is machine-generated.

Scanless two-photon voltage imaging allows high-resolution neural recordings. This technique enables simultaneous voltage imaging and photostimulation for mapping brain circuits.

Area of Science:

Neuroscience
Optical Imaging
Optogenetics

Background:

All-optical neurophysiology enables simultaneous photostimulation and recording.
Scanless two-photon photostimulation allows targeting multiple neurons.

Approach:

Demonstrated scanless two-photon voltage imaging with high resolution and contrast.
Utilized soma-targeted genetically encoded voltage indicator JEDI-2P-kv.
Characterized imaging performance with different lasers and repetition rates.

Key Points:

Achieved high-frequency spike train and sub-threshold depolarization recordings in intact brain tissue.
Performed simultaneous recordings from up to ten neurons using a low repetition-rate laser.
Co-expressed JEDI-2P-kv and channelrhodopsin ChroME-ST for combined imaging and stimulation.

Keywords:

Two-photon microscopy computer-generated holography optogenetics temporal focusing voltage imaging

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Conclusions:

Enabled in-situ validation of light-evoked action potentials.
Paves the way for rapid and scalable identification of functional neural connections.
Advances all-optical methods for neurophysiology research.