Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of vitamin D on the colon cancer immune microenvironment: results of a randomized clinical trial of preoperative vitamin D supplementation in patients with stage I-III colon cancer.

Cancer discovery·2026
Same author

Local and circulating cytotoxic CD4⁺ T cells are early markers of disease activity in pediatric Crohn's disease.

medRxiv : the preprint server for health sciences·2026
Same author

Accurate trajectory inference in time-series spatial transcriptomics with structurally-constrained optimal transport.

Nature communications·2026
Same author

Aging disrupts spatiotemporal coordination in the cycling murine ovary.

Nature aging·2026
Same author

Phenylalanine Versus Tyrosine (Pos. 367/332 in MCT1/MCT4) in the Substrate Binding Site Defines Affinity and Preferred Directionality of Human Monocarboxylate Transporters 1-4.

Acta physiologica (Oxford, England)·2026
Same author

SwitchCraft: A Programmatic Framework for Designing State-Switching Proteins.

ArXiv·2026

Related Experiment Video

Updated: Aug 11, 2025

Development of automated imaging and analysis for zebrafish chemical screens.
10:49

Development of automated imaging and analysis for zebrafish chemical screens.

Published on: June 24, 2010

11.5K

Compressed phenotypic screens for complex multicellular models and high-content assays.

Benjamin E Mead1,2,3,4, Conner Kummerlowe1,2,3,4,5, Nuo Liu1,2,3,4,5

  • 1Institute for Medical Engineering and Science (IMES), Department of Chemistry, Massachusetts Institute of Technology; Cambridge, MA, 02139, USA.

Biorxiv : the Preprint Server for Biology
|February 7, 2023
PubMed
Summary
This summary is machine-generated.

We developed a method to compress high-throughput phenotypic screens by pooling perturbations and using computational deconvolution, increasing efficiency for drug discovery and basic biology research.

More Related Videos

A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications
06:40

A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications

Published on: December 28, 2021

3.4K
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

7.9K

Related Experiment Videos

Last Updated: Aug 11, 2025

Development of automated imaging and analysis for zebrafish chemical screens.
10:49

Development of automated imaging and analysis for zebrafish chemical screens.

Published on: June 24, 2010

11.5K
A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications
06:40

A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications

Published on: December 28, 2021

3.4K
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

7.9K

Area of Science:

  • Biotechnology
  • Drug Discovery
  • Genomics

Background:

  • High-throughput phenotypic screens are crucial for therapeutic discovery but limited by scale.
  • Complex multicellular models and high-content readouts offer rich biological insights.
  • Current screening methods face challenges in efficiency and scalability.

Approach:

  • Developed a computational deconvolution method for pooled perturbations in phenotypic screens.
  • Validated the compressed screening approach using small molecule libraries and high-content imaging.
  • Applied compressed screening to analyze transcriptional responses in pancreatic cancer organoids to tumor microenvironment ligands.

Key Points:

  • Compressed screening significantly increases experimental efficiency compared to conventional methods.
  • Identified reproducible phenotypic shifts in patient-derived pancreatic cancer organoids.
  • Uncovered ligand-induced transcriptional responses correlating with clinical features and distinct from public databases.

Conclusions:

  • This novel approach enhances the scalability of phenotypic screens using complex models and rich readouts.
  • Enables more efficient drug discovery and advances fundamental biological research.
  • Facilitates the interrogation of cellular responses for translational medicine applications.