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Xiaotian Wang1,2, Jiahao Niu1,2, Yi Yang1

  • 1School of Life Sciences, State Key Laboratory of Membrane Biology, Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing, China.

Chemical Communications (Cambridge, England)
|February 7, 2023
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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...