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Related Experiment Video

Updated: Aug 11, 2025

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
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Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System.

Yu-Xin Luo1, Yang Yu1,2

  • 1Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.

Methods in Molecular Biology (Clifton, N.J.)
|February 7, 2023
PubMed
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Human embryonic stem cell-derived blastoids (EPS-blastoids) offer a novel model for studying early human development and disease. This protocol details their efficient generation and characterization in vitro.

Area of Science:

  • Developmental Biology
  • Stem Cell Research
  • Embryogenesis

Background:

  • Stem cell-derived embryos (blastoids) are crucial for studying early human development in vitro.
  • Human embryonic stem cells (EPS cells) are a promising resource for blastoid generation due to their lineage contribution.
  • Existing methods for blastoid generation have varying efficiencies and complexities.

Purpose of the Study:

  • To develop an efficient three-dimensional, two-step induction system for generating human blastoids from EPS cells.
  • To characterize the developmental fidelity and lineage contribution of EPS-blastoids.
  • To provide a detailed protocol for the generation, maintenance, and phenocopying of human EPS blastoids.

Main Methods:

  • Utilized a three-dimensional, two-step induction system.
Keywords:
BlastoidsEPS cellsEmbryogenesisStem celliPSCs

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Last Updated: Aug 11, 2025

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  • Employed human embryonic stem cells (EPS cells) as the starting material.
  • Performed morphological and transcriptomic analyses for characterization.

    • Main Results:

    • Successfully generated human blastoids (EPS-blastoids) from EPS cells.
    • EPS-blastoids recapitulated key developmental processes and cell lineages of natural human blastocysts.
    • Extended in vitro culture of EPS-blastoids yielded postimplantation embryonic structures.

    Conclusions:

    • The developed protocol provides an efficient method for generating human EPS blastoids.
    • EPS-blastoids serve as a valuable in vitro model for human embryogenesis and developmental biology research.
    • This system facilitates applications in developmental disease modeling and drug discovery.