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Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells:

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Related Experiment Video

Updated: Aug 11, 2025

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Published on: May 8, 2020

Lineage Tracing by Single-Cell Transcriptomics Decoding Dynamics of Lineage Commitment.

Ping Yu¹, Lin Cheng²

¹Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Methods in Molecular Biology (Clifton, N.J.)

|February 7, 2023

Summary

This summary is machine-generated.

This study presents a protocol for lineage tracing using single-cell transcriptomics, enabling detailed observation of cell development. This method clarifies the gradual changes in transcription factor levels during cell lineage commitment.

Keywords:

Cell fate Development Lineage commitment Lineage tracing Single-cell transcriptomics

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Area of Science:

* Developmental Biology
* Molecular Biology
* Genomics

Background:

* Understanding cell fate and progeny is crucial for stem cell and cancer research.
* Lineage commitment involves gradual, continuous changes in transcription factor levels.
* Single-cell sequencing technologies offer high-resolution insights into cellular states.

Purpose of the Study:

* To introduce a detailed protocol for lineage tracing via single-cell transcriptomics.
* To elucidate the dynamics of cell lineage commitment.
* To provide a method for tracking individual cell fates and their developmental trajectories.

Main Methods:

* Development and application of a single-cell transcriptomics protocol.
* Analysis of gene expression profiles at single-cell resolution.
* Integration of transcriptomic data for lineage tracing.

Main Results:

* Demonstrated the feasibility of lineage tracing using single-cell transcriptomics.
* Characterized the continuous changes in transcription factor dynamics during lineage commitment.
* Provided a high-resolution view of cell state transitions.

Conclusions:

* Single-cell transcriptomics is a powerful tool for lineage tracing.
* The developed protocol clarifies the dynamics of cell lineage commitment.
* This approach advances stem cell and cancer research by enabling detailed cell fate analysis.