Related Experiment Video

Updated: Aug 11, 2025

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

6.8K

SRSF7 and SRSF3 depend on RNA sequencing motifs and secondary structures to regulate Microprocessor

Minh Ngoc Le1, Trung Duc Nguyen1, Tuan Anh Nguyen2

  • 1Division of Life Science, The Hong Kong University of Science & Technology, Hong Kong, China.

Life Science Alliance
|February 7, 2023
PubMed

Insights

SRSF7 and SRSF3 enhance Microprocessor cleavage of pri-miRNAs by recognizing specific motifs and secondary structures, ensuring accurate miRNA biogenesis and gene regulation.

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Gene Regulation

Background:

  • Microprocessor complex initiates microRNA (miRNA) biogenesis by cleaving precursor miRNAs (pri-miRNAs).
  • Cofactors like SRSF3 assist Microprocessor in cleaving pri-miRNAs, but the full mechanisms and roles of other factors remain unclear.
  • The function of SRSF7, a paralog of SRSF3, in miRNA biogenesis is largely undiscovered.

Purpose of the Study:

  • To investigate the role of SRSF7 in miRNA biogenesis.
  • To elucidate the molecular mechanisms by which SRSF7 and SRSF3 enhance Microprocessor cleavage.
  • To identify sequence motifs and structural requirements for cofactor-assisted Microprocessor activity.

Main Methods:

  • High-throughput pri-miRNA cleavage assays were performed.
  • Assays involved Microprocessor complex, SRSF7, and SRSF3.
  • Analysis of cofactor interactions with pri-miRNA motifs and secondary structures in human cells.

Main Results:

  • SRSF7 was demonstrated to stimulate Microprocessor cleavage.
  • Both SRSF7 and SRSF3 were shown to function with CRC and CNNC motifs.
  • These motifs require specific secondary structures for cofactor-mediated cleavage.
  • SRSF7 and SRSF3 influence Microprocessor cleavage sites within human cells.

Conclusions:

  • SRSF7 plays a significant role in miRNA biogenesis.
  • SRSF7 and SRSF3 enhance Microprocessor activity through specific motif recognition and structural requirements.
  • These findings provide a comprehensive understanding of the molecular mechanisms governing Microprocessor-cofactor interactions in miRNA processing.

More Related Videos

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.0K
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.4K

Related Concept Videos

Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
64
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.9K
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
64.3K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.3K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
74
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.2K