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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

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Rotors tailoring molecular stacking for constructing multi-stimuli-responsive luminescent materials

Zheng-Fei Liu1, Lan Zeng1, Li-Ya Niu1

  • 1Key Laboratory of Radiopharmaceuticals, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China. qzyang@bnu.edu.cn.

Chemical Communications (Cambridge, England)
|February 8, 2023
PubMed

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.