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Updated: Aug 11, 2025

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

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Published on: September 19, 2017

Integrating machine learning and electrochemistry to develop a glucose biosensor assembled with Ganoderma applanatum

Vanessa E Abrantes-Coutinho¹, André O Santos¹, Brenna E B Holanda¹

¹Centro de Ciência e Tecnologia, Universidade Federal do Cariri, 63048-080 Juazeiro do Norte, CE, Brazil.

Bioelectrochemistry (Amsterdam, Netherlands)

|February 8, 2023

Summary

This study introduces a novel fungal lectin biosensor for glucose detection. The Ganoderma applanatum lectin (GAL) biosensor demonstrates high sensitivity and accuracy, enabling glucose quantification in pharmaceutical products.

Keywords:

Electrochemical biosensor Ganoderma applanatum Glucose Lectins Macrofungi

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Area of Science:

Biochemistry
Electrochemistry
Biosensor Technology

Background:

Fungal lectins offer significant biotechnological promise but require further characterization for optimal application.
Limited understanding of lectin properties hinders their use in biosensing platforms.

Purpose of the Study:

To develop and optimize a glucose biosensor utilizing Ganoderma applanatum lectin (GAL).
To establish ideal electroanalytical conditions for GAL immobilization and performance using machine learning and molecular modeling.

Main Methods:

Machine learning identified optimal conditions for GAL electroanalysis.
Lectin immobilization on a Prussian blue-modified glassy carbon electrode (GAL/ta-PB/GCE).
Electrochemical characterization and performance evaluation of the developed biosensor.

Main Results:

GAL exhibits moderate stability across pH (4-8) and temperature (20-60 °C) ranges.
The GAL/ta-PB/GCE biosensor achieved a low detection limit (10.2 pM) and high sensitivity (0.012 µA µM⁻¹cm⁻²).
The biosensor successfully quantified glucose in pharmaceutical formulations.

Conclusions:

The integration of theoretical and experimental approaches facilitates advancements in bioelectrochemical studies.
Ganoderma applanatum lectin is a viable glucose biorecognition element for sensitive biosensor development.