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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Related Experiment Video

Updated: Aug 11, 2025

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
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Cryopreservation: A Comprehensive Overview, Challenges, and Future Perspectives.

Arpana Parihar1, Avinash Kumar2, Udwesh Panda2

  • 1Industrial Waste Utilization, Nano and Biomaterials, CSIR-Advanced Materials and Processes Research Institute (AMPRI), Hoshangabad Road, Bhopal, Madhya Pradesh, 462026, India.

Advanced Biology
|February 9, 2023
PubMed
Summary
This summary is machine-generated.

Cryopreservation preserves cells long-term using freezing, storage, and thawing. Advances minimize cellular damage, enhancing cell revival and clinical applications, especially for stem cells.

Keywords:
cryoinjurycryoprotectantsstem cells preservationultrarapid cryopreservation

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Area of Science:

  • Cell Biology
  • Biotechnology
  • Clinical Applications

Background:

  • Cryopreservation is the primary method for long-term cell preservation.
  • Effective cryopreservation requires optimal freezing, storage, and thawing protocols.
  • Minimizing cellular damage during sample processing is crucial for viability.

Purpose of the Study:

  • To review fundamental cryopreservation techniques and their clinical implementation.
  • To discuss advancements in cryopreservation, including cryoprotectants and procedures.
  • To highlight applications in stem cell preservation and future scope.

Main Methods:

  • Description of standard cryopreservation procedures (slow freezing, vitrification).
  • Discussion of membrane-permeating and non-permeating cryoprotectants.
  • Review of variables influencing the cryopreservation process and resuscitation.

Main Results:

  • Recent advances minimize cellular damage during cryopreservation.
  • Various techniques enhance cell preservation output and minimize cryoinjury.
  • Improved cell revival rates are achievable through optimized processes.

Conclusions:

  • Cryopreservation techniques are vital for clinical settings, particularly for stem cells.
  • Understanding and implementing advanced methods improve cell viability and revival.
  • Future scope includes overcoming challenges and further enhancing cryopreservation efficacy.