[Spectrophotometric method of determining the osmotic resistance of lysosomes isolated from animal tissue]

Rapid spectrophotometric determination of animal tissue lysosome osmoresistance is described. The duration of analysis is decreased by 5-6 h, as compared to enzymatic method. The process of lysosome degradation was linear at 0.7 M sucrose concentration. The dependence of sucrose molarity on the extent of lysosome lysis was complex. The lysosome lysis rate rapidly increased at sucrose molarity less than 0.7 M. Lysosome incubation at 0.4 degrees C for 24 h increased their osmotic sensitivity within the whole investigated osmotic pressure range. Isolated lysosome osmoresistance may be used as an index of dynamic structural and functional state of these particles and the extent of their degradation caused by various physical and chemical factors in vivo and in vitro. The measure of the initial absorption (A520) and the initial lysosome lysis rate (delta A520/min), as well as biological half-life may be the index of such an effect.