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Screening of Active Ingredients from Wendan Decoction in Alleviating Palmitic Acid-Induced Endothelial Cell Injury
Nan Xu1,2, Muhammad Ijaz1, Haiyan Shi3,4
1Department of Pharmacology, School of Pharmaceutical Science, Shandong University, Jinan 250012, China.
Molecules (Basel, Switzerland)
|February 11, 2023
Summary
Wendan Decoction (WDD) protects against vascular endothelial cell injury, potentially through active compounds like trigonelline and quercetin. This study identifies key ingredients contributing to its therapeutic effects in hyperlipidemia treatment.
Area of Science:
- Pharmacology and Traditional Chinese Medicine (TCM)
- Cardiovascular Research
- Cell Biology
Background:
- Traditional Chinese Medicine (TCM) is vital for treating various diseases.
- Wendan Decoction (WDD), a classic TCM formula, shows promise for hyperlipidemia.
- Hyperlipidemia-induced cardiovascular injury necessitates understanding WDD's anti-vascular endothelial cell injury (AVECI) mechanisms.
Purpose of the Study:
- To investigate the protective effects of Wendan Decoction (WDD) on vascular endothelial cells.
- To identify potential pharmacodynamic substances responsible for WDD's anti-vascular endothelial cell injury (AVECI) activity.
Main Methods:
- Chemical constituents of WDD were identified using Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS).
- Protective effects of 16 WDD batches on human umbilical vein endothelial cells (HUVECs) were evaluated.
- Gray Relation Analysis (GRA) and Partial Least Squares Regression (PLSR) correlated chemical ingredients with AVECI.
Main Results:
- WDD demonstrated a significant protective effect on endothelial cells.
- Marked discrepancies in pharmacological properties were observed across 16 WDD batches.
- Trigonelline, liquiritin, hesperidin, hesperetin, scopoletin, morin, quercetin, isoliquiritigenin, liquiritigenin, and formononetin were identified as potential AVECI active ingredients.
Conclusions:
- Wendan Decoction (WDD) effectively protects against palmitic acid-induced endothelial cell injury.
- The protective effect is likely linked to its specific chemical component content.
- The employed analytical method is suitable for discovering active components in classical TCM formulations.
