A bacterial cold-active dye-decolorizing peroxidase from an Antarctic Pseudomonas strain
Célica Cagide1, Juan José Marizcurrena1, Diego Vallés2
1Sección Bioquímica, Instituto de Biología, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400, Montevideo, Uruguay.
Applied Microbiology and Biotechnology
|February 11, 2023
Summary
An Antarctic microbe yields a cold-active dye-decolorizing peroxidase (DyP). This enzyme, rDyP-AU10, shows high activity at low temperatures and potential for bioremediation and industrial applications.
Area of Science:
- Biochemistry
- Enzymology
- Microbiology
Background:
- Dye-decolorizing peroxidases (DyPs) are hemeproteins involved in H2O2-dependent oxidation and lignin degradation.
- DyPs from mesophilic and thermophilic organisms have been characterized, but cold-active variants are less understood.
Purpose of the Study:
- To identify and characterize a novel dye-decolorizing peroxidase from an Antarctic cold-tolerant microbe.
- To investigate the biochemical properties and potential biotechnological applications of the recombinant enzyme.
Main Methods:
- Gene identification from Antarctic Pseudomonas sp. AU10 genome.
- Recombinant protein expression in Escherichia coli and purification.
- Enzyme activity assays using ABTS, hydroquinone, and ascorbate.
- Steady-state and stopped-flow kinetic analyses.
- Dye decolorization and kraft lignin modification assays.
Main Results:
- A class B DyP gene (dyp) was identified in Pseudomonas sp. AU10.
- Recombinant rDyP-AU10 was successfully produced and purified, primarily as a dimer.
- rDyP-AU10 exhibited high enzymatic activity at low temperatures (20 °C) and thermo-instability, characteristic of psychrophilic enzymes.
- Kinetic studies revealed efficient Compound I formation and turnover.
- The enzyme demonstrated decolorization of dyes and modification of kraft lignin.
Conclusions:
- The Antarctic Pseudomonas sp. AU10 harbors a cold-active DyP with unique characteristics.
- rDyP-AU10 shows promise for applications in bioremediation, and the cellulose and biofuel industries due to its low-temperature activity and substrate modification capabilities.
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