Genome Editing in Mouse and Rat by Electroporation

Area of Science:

• Genetics
• Molecular Biology
• Animal Science

Background:

• Genome editing in rodents is crucial for biological research.
• Traditional methods for generating genome-edited rodents can be time-consuming and inefficient.
• Engineered nucleases like ZFN, TALEN, and CRISPR-Cas9 have revolutionized genome editing.

Purpose of the Study:

• To present the latest technical information on producing genome-edited mouse and rat strains.
• To highlight the efficiency and advantages of the TAKE method for generating genetically modified rodents.
• To provide a comprehensive overview of CRISPR-Cas9 application in rodent strain production.

Main Methods:

• Utilizing the Technique for Animal Knockout system by Electroporation (TAKE).
• Direct introduction of engineered nucleases (CRISPR-Cas9) into intact rodent embryos via electroporation.
• Focus on efficient and rapid generation of new genome-edited strains.

Main Results:

• The TAKE method significantly accelerates the production of genome-edited mouse and rat strains.
• CRISPR-Cas9 technology enables easy, rapid, and high-efficiency genome editing.
• Successful application of TAKE for generating novel rodent models.

Conclusions:

• The TAKE method, combined with CRISPR-Cas9, offers a powerful and efficient approach for generating genome-edited rodents.
• This technique streamlines the creation of valuable animal models for scientific research.
• Latest advancements in rodent genome editing are presented, emphasizing practical applications.