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Related Experiment Video

Updated: Aug 10, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

Genome Editing of C. elegans.

¹Molecular Neuroscience Research Center, Shiga University of Medical Science, Otsu, Shiga, Japan. sugit@hiroshima-u.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)

|February 11, 2023

Summary

Caenorhabditis elegans is a model organism for genetic studies. This protocol details genome editing techniques for this nematode, enhancing genetic analysis capabilities.

Area of Science:

Molecular Biology
Genetics
Developmental Biology

Background:

Caenorhabditis elegans is a widely used model organism in biological research.
Its key features include a rapid life cycle, transparent body, and well-annotated genome.
Genome editing is increasingly employed to advance genetic studies in C. elegans.

Purpose of the Study:

To introduce a commonly utilized protocol for genome editing in C. elegans.
To facilitate advanced genetic analyses in this model organism.

Main Methods:

The protocol focuses on established genome editing techniques applicable to C. elegans.
Specific methods for precise genetic modification are detailed.

Main Results:

Keywords:

C. elegans CRISPR/Cas9 Homology-dependent repair

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Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

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Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

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Published on: September 25, 2013

The presented protocol enables efficient genome editing in C. elegans.
This facilitates targeted genetic investigations and functional studies.

Conclusions:

Genome editing protocols are crucial for advancing genetic research in C. elegans.
This method enhances the utility of C. elegans as a model organism for diverse biological studies.