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Determination of erythrocyte protoporphyrin by magnetic circular dichroism
1Department of Medical Biochemistry, University of Cape Town Medical School, Observatory, South Africa.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|October 15, 1987
Abstract:
A method for the determination of erythrocyte protoporphyrin by magnetic circular dichroism is reported. The method was sufficiently sensitive to distinguish normal from elevated levels of erythrocyte protoporphyrin and may be useful for diagnostic screening purposes. Erythrocyte protoporphyrin levels determined by magnetic circular dichroism correlated well with levels determined by the fluorimetric method of Piomelli, viz. a plot of MCD versus fluorimetry yielded a straight line relationship with a slope of 0.98 +/- 0.01, y-intercept of 0.05 +/- 0.35 and correlation coefficient of 0.99.