A BODIPY-Based Far-Red-Absorbing Fluorescent Probe for Hypochlorous Acid Imaging.

Area of Science:

• Biomedical Engineering
• Chemical Biology
• Analytical Chemistry

Background:

• Hypochlorous acid (HClO) is a reactive oxygen species produced by white blood cells.
• Elevated intracellular HClO levels are linked to chronic cardiovascular and nervous system diseases.
• Accurate monitoring of HClO in biological samples is crucial for understanding its role in disease.

Purpose of the Study:

• To develop a far-red fluorescent probe for sensitive and selective detection of hypochlorous acid (HClO).
• To create a ratiometric probe for accurate quantification of HClO in biological samples.
• To evaluate the probe's performance in vitro using confocal microscopy.

Main Methods:

• Synthesis of a novel far-red fluorescent probe based on BODIPY chromophore and aldoxime sensing group.
• Spectroscopic analysis to characterize the probe's optical properties and response to HClO.
• In vitro experiments using confocal microscopy to visualize and quantify HClO in biological samples.

Main Results:

• The developed probe exhibits strong absorption in the far-red region, minimizing light toxicity and enhancing tissue penetration.
• The probe displays a ratiometric response, with a significant increase in emission ratio (I645/I670) upon HClO detection.
• Visual detection of color change and accurate ratiometric analysis of HClO concentrations were achieved.
• Successful demonstration of HClO monitoring in bio-samples using confocal microscopy.

Conclusions:

• A novel far-red ratiometric fluorescent probe for hypochlorous acid (HClO) has been successfully developed.
• The probe enables sensitive, selective, and accurate detection of HClO in biological samples.
• This probe holds potential for in vitro diagnostics and research into HClO-related diseases.