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Flavylium- and Silylrhodapolymethines In Excitation Multiplexing.
1Department of Chemistry, Texas A&M University, Box 39912, College Station, Tx 77842 (USA).
Chemphotochem
|March 13, 2023
Summary
Researchers developed new flavylium dyes for near-infrared II imaging. This method uses selective excitation for multiplexing, a shift from traditional emission-based techniques.
Area of Science:
- Biophotonics
- Organic Chemistry
Background:
- Multiplexing in bioimaging typically relies on discriminating distinct emission wavelengths.
- Existing near-infrared II (NIR-II) fluorophores have limitations for advanced multiplexing strategies.
Purpose of the Study:
- To develop a novel multiplexing strategy for NIR-II imaging using selective excitation.
- To create and evaluate flavylium polymethine dyes for NIR-II applications.
Main Methods:
- Synthesized a set of flavylium polymethine dyes.
- Selected two fluorophores compatible with common NIR-II excitation lasers.
- Employed selective excitation for multiplexing, capturing broad emission in the NIR-II region.
Main Results:
- Successfully created flavylium dyes enabling NIR-II excitation.
- Demonstrated a new paradigm of multiplexing based on selective excitation rather than emission discrimination.
- Identified a requirement for discrete, water-soluble fluorophores, including one activated at 808 nm.
Conclusions:
- Excitation multiplexing with flavylium dyes offers a novel approach for NIR-II bioimaging.
- This strategy represents a significant shift from conventional emission-based multiplexing.
- Further development is needed to optimize the discrete water-soluble fluorophores for this technique.
