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Updated: Aug 10, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

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Construction of PARPi Resistance-related Competing Endogenous RNA Network

Lili Kong1, Jiaqi Xu2,3, Lijun Yu1

  • 1Beijing Rehabilitation Hospital, Capital Medical University, Beijing, China.

Current Genomics
|February 13, 2023
PubMed

Insights

Poly (ADP-ribose) polymerase inhibitors (PARPi) resistance in ovarian cancer is a challenge. This study identified key RNA networks and pathways, revealing potential new targets for overcoming PARPi resistance.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Ovarian cancer is a common gynecological malignancy.
  • Poly (ADP-ribose) polymerase inhibitors (PARPi) are used for treatment, but resistance limits their efficacy.
  • Mechanisms underlying PARPi resistance require further elucidation.

Purpose of the Study:

  • To characterize RNA expression profiles and construct regulatory networks in PARPi-resistant ovarian cancer cells.
  • To identify key regulatory ceRNA axes and their involved pathways in PARPi resistance.
  • To provide insights for developing novel therapeutic targets against PARPi resistance.

Main Methods:

  • RNA sequencing was performed on PARPi (Olaparib)-induced ovarian cancer cells.
  • Differential expression analysis of mRNAs, lncRNAs, circRNAs, and miRNAs was conducted.
  • RNA regulatory networks, particularly ceRNA networks, were constructed and analyzed.

Main Results:

  • Differentially expressed genes were enriched in PI3K/AKT, MAPK signaling, and metabolic pathways.
  • Key regulatory ceRNA axes involving lncRNAs, circRNAs, miRNAs, and mRNAs were identified.
  • miR-320b was identified as a crucial mediator in these regulatory networks.

Conclusions:

  • Key regulatory lncRNA-circRNA-miRNA-mRNA axes and their associated pathways were revealed in PARPi-resistant ovarian cancer.
  • These findings offer new perspectives on ceRNA regulatory networks in drug resistance.
  • The identified axes and pathways represent potential targets for overcoming PARPi resistance in ovarian cancer.
Keywords:
DNAOvarian cancerPARPi resistanceSSBceRNA networkmRNA

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