Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biological Effects of Radiation02:59

Biological Effects of Radiation

15.8K
All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
15.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Suicidal behaviour in adolescents with affective disorders: A study in a crisis intervention unit (CIU).

PloS one·2025
Same author

Thoracic ectopia cordis: A case report.

Medwave·2024
Same author

Instruments of Child-to-Parent Violence: Systematic Review and Meta-Analysis.

Healthcare (Basel, Switzerland)·2023
Same author

Designing Effective Multi-Target Drugs and Identifying Biomarkers in Recurrent Pregnancy Loss (RPL) Using In Vivo, In Vitro, and In Silico Approaches.

Biomedicines·2023
Same author

Exploring Probenecid Derived 1,3,4-Oxadiazole-Phthalimide Hybrid as α-Amylase Inhibitor: Synthesis, Structural Investigation, and Molecular Modeling.

Pharmaceuticals (Basel, Switzerland)·2023
Same author

Dietary Thymol Improved Growth, Body Composition, Digestive Enzyme Activities, Hematology, Immunity, Antioxidant Defense, and Resistance to <i>Streptococcus iniae</i> in the Rainbow Trout (<i>Oncorhynchus mykiss</i>).

Aquaculture nutrition·2023

Related Experiment Video

Updated: Aug 10, 2025

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse
08:27

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse

Published on: July 25, 2011

19.8K

Protective Potentials of Alpha-Lipoic Acid against Ionizing Radiation-Induced Brain Damage in Rats.

Ji Xu1, Ameer A Alameri2, Rahman S Zabibah3

  • 1Department of Rehabilitation Medicine, 3201 Hospital, Hanzhong 723000, China.

Oxidative Medicine and Cellular Longevity
|February 13, 2023
PubMed
Summary

Alpha-lipoic acid pretreatment mitigates ionizing irradiation-induced brain damage in rats. This antioxidant therapy reduced oxidative stress markers and apoptosis, offering neuroprotective effects against radiation exposure.

More Related Videos

A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats
10:15

A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats

Published on: November 6, 2019

8.4K
Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition
11:45

Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition

Published on: November 14, 2013

12.2K

Related Experiment Videos

Last Updated: Aug 10, 2025

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse
08:27

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse

Published on: July 25, 2011

19.8K
A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats
10:15

A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats

Published on: November 6, 2019

8.4K
Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition
11:45

Functional Interrogation of Adult Hypothalamic Neurogenesis with Focal Radiological Inhibition

Published on: November 14, 2013

12.2K

Area of Science:

  • Neuroscience
  • Radiology
  • Biochemistry

Background:

  • Ionizing radiation exposure can induce significant oxidative damage and apoptosis in brain tissue.
  • Oxidative stress is a key mechanism underlying radiation-induced brain injury.
  • Alpha-lipoic acid is a potent antioxidant with potential therapeutic properties.

Purpose of the Study:

  • To investigate the neuroprotective effects of alpha-lipoic acid against ionizing irradiation-induced oxidative damage and apoptosis in a rat brain model.
  • To evaluate the impact of alpha-lipoic acid on key oxidative stress biomarkers and apoptotic markers following radiation exposure.

Main Methods:

  • Rats were pretreated with alpha-lipoic acid (200 mg/kg) for one week before single-dose whole-brain X-radiation (15 Gy).
  • Biochemical assays measured malondialdehyde (MDA), nitric oxide, catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in brain tissue.
  • Western blot analysis assessed protein expression of NOX2, NOX4, and caspase-3.

Main Results:

  • Irradiation significantly increased MDA and nitric oxide levels, while alpha-lipoic acid pretreatment significantly reduced these markers.
  • Radiation exposure decreased endogenous antioxidant enzyme activities (CAT, SOD, GPx), which were restored by alpha-lipoic acid treatment.
  • Protein expression of NOX2, NOX4, and caspase-3 was significantly elevated post-irradiation, but suppressed by alpha-lipoic acid pretreatment.

Conclusions:

  • Alpha-lipoic acid pretreatment demonstrates significant neuroprotective effects against ionizing irradiation-induced brain damage in rats.
  • The findings suggest alpha-lipoic acid mitigates radiation-induced oxidative stress and apoptosis through modulation of key molecular pathways.