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Updated: Aug 10, 2025
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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
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Metagenome assembled genomes are for eukaryotes too
Ramon Massana1, David López-Escardó1
1Institut de Ciències del Mar (ICM-CSIC), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Catalonia, Spain.
Cell Genomics
|February 13, 2023
Summary
Environmental sequencing successfully generated hundreds of eukaryotic MAGs from marine plankton, challenging prior assumptions. This breakthrough provides valuable genomic and functional insights into eukaryotic microbial communities.
Area of Science:
- Marine biology
- Genomics
- Metagenomics
Background:
- Environmental community sequencing is widely used for prokaryotic MAGs.
- There is a prevailing notion that eukaryotic MAGs are difficult to obtain via this method.
Discussion:
- Delmont et al. processed a large-scale marine plankton sequencing dataset.
- The study successfully generated 683 eukaryotic MAGs.
- Functional information was also extracted from the MAGs.
Key Insights:
- This research demonstrates the feasibility of producing eukaryotic MAGs from environmental sequencing data.
- The study overcomes previous limitations in eukaryotic metagenome assembly.
- A significant number of high-quality eukaryotic MAGs were recovered.
Outlook:
- This work opens new avenues for studying eukaryotic microbial diversity and function in various environments.
- Future research can leverage these methods to explore underrepresented eukaryotic lineages.
- The findings will advance our understanding of marine plankton ecosystems.
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