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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Aug 10, 2025

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Platelet Phenotyping by Full Spectrum Flow Cytometry.

Benjamin E J Spurgeon¹, Andrew L Frelinger¹

¹Center for Platelet Research Studies, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Harvard Medical School, Boston, Massachusetts.

Current Protocols

|February 13, 2023

Summary

This summary is machine-generated.

Full spectrum flow cytometry provides detailed analysis of platelet function and phenotype, offering advantages over traditional methods for studying disease biology and discovering novel platelet characteristics.

Keywords:

biomarkers blood platelets flow cytometry phenotype platelet activation

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Last Updated: Aug 10, 2025

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

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Published on: June 10, 2025

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

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Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

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Published on: November 29, 2024

Area of Science:

Hematology
Immunology
Biotechnology

Background:

Platelets are crucial for hemostasis, immunity, and inflammation.
Assessing platelet phenotype and function aids in understanding disease biology and pathology.
Standard tests for platelet analysis have limitations.

Purpose of the Study:

To describe the analysis of platelets using full spectrum flow cytometry.
To highlight the advantages of full spectrum flow cytometry for platelet studies.
To provide guidance on controls and interpretation of results.

Main Methods:

Utilized full spectrum flow cytometry for platelet analysis.
Included detailed protocols for platelet phenotyping.
Described methods for spectral unmixing and data preprocessing.

Main Results:

Full spectrum flow cytometry enables sensitive, simultaneous detection of multiple platelet biomarkers.
The assay provides comprehensive information on platelet biology.
It allows assessment of in vivo activation, in vitro reactivity, and discovery of novel phenotypes.

Conclusions:

Full spectrum flow cytometry is a powerful tool for in-depth platelet analysis.
This technique offers significant advantages for research in hemostasis, immunity, and inflammation.
The described methods facilitate robust interpretation of platelet data.