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Updated: Aug 10, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
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A Guide to Sequencing for Long Repetitive Regions

Nobuaki Kono1

  • 1Institute for Advanced Biosciences, Keio University, Tsuruoka City, Yamagata, Japan. ciconia@sfc.keio.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|February 13, 2023
PubMed
Summary

Analyzing genes with repetitive DNA sequences is difficult due to assembly and accuracy issues. This study presents a novel strategy combining overlap-layout-consensus and long-read scaffolding to accurately determine the full length of these challenging repeats.

Keywords:
De novo sequencingHighly repetitive sequenceNon-model organismOverlap-layout-consensusStructural protein

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