Comprehensive Targeted Lipidomic Profiling for Research and Clinical Applications

Area of Science:

• Biochemistry
• Analytical Chemistry
• Clinical Diagnostics

Background:

• Mass spectrometry is a key technique for lipidome analysis.
• Current methods face limitations in balancing lipidome coverage, structural detail, and sample throughput.
• Biomedical research requires analyzing large numbers of human samples due to individual variations.

Purpose of the Study:

• To develop a targeted lipidomics workflow for high-throughput, reproducible analysis of human plasma.
• To maintain detailed structural information and broad lipidome coverage in large-scale studies.
• To facilitate association studies between lipid profiles and clinical outcomes.

Main Methods:

• Development of a targeted lipidomics workflow.
• Application to profiling thousands of human plasma samples.
• Emphasis on reproducibility, structural detail, and lipidome coverage.

Main Results:

• Successful reproducible profiling of thousands of plasma samples.
• Achieved a balance between lipidome coverage and structural detail.
• Demonstrated a systematic approach for large-scale lipidomic analysis.

Conclusions:

• The described targeted lipidomics workflow is suitable for large-scale human plasma sample analysis.
• This approach supports robust identification of lipid-biomarker associations in clinical research.
• The workflow addresses key limitations in current lipidomic analysis for biomedical applications.