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Related Concept Videos

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Related Experiment Video

Updated: Aug 9, 2025

Use of Stopped-Flow Fluorescence and Labeled Nucleotides to Analyze the ATP Turnover Cycle of Kinesins
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A Small-Molecule Fluorescence Probe for Nuclear ATP.

Umme Tamima1, Sourav Sarkar1, Md Reyazul Islam2

  • 1Department of Chemistry, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk, 37673 (Republic of, Korea.

Angewandte Chemie (International Ed. in English)
|February 15, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel small-molecule probe for detecting nuclear adenosine triphosphate (ATP). This probe shows significantly higher nuclear ATP levels in cancer cells and tissues, offering potential for studying nuclear ATP-related biology.

Keywords:
BenzocoumarinNuclear ATPSmall-Molecule Fluorescent ProbeTumor ATP LevelsTwo-Photon Imaging

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Fluorescence monitoring of adenosine triphosphate (ATP) in organelles is limited by biosensor sensitivity, biocompatibility, and accessibility.
  • Existing small-molecule ATP probes often fail to target the nucleus, hindering nuclear ATP research.

Purpose of the Study:

  • To develop the first small-molecule probe for selective detection of nuclear ATP.
  • To investigate the potential of this probe for studying nuclear ATP-associated biology.

Main Methods:

  • Development of a novel small-molecule probe with reversible binding for selective nuclear ATP detection.
  • Characterization of probe performance, including fluorescence enhancement and selectivity against biological species.
  • Application of the probe to measure nuclear ATP levels in cancerous and normal cell lines and tumor tissues.

Main Results:

  • The probe exhibits a 25-fold fluorescence enhancement at pH 7.4 with excellent selectivity.
  • Nuclear ATP levels were found to be 2.1-3.3-fold higher in cancerous cell lines and 3.9-7.8-fold higher in tumor tissues compared to normal counterparts.
  • Elevated nuclear ATP levels correlate with the mitosis phase in cells.

Conclusions:

  • A novel small-molecule probe enables selective fluorescence monitoring of nuclear ATP.
  • The probe reveals significantly higher nuclear ATP concentrations in cancer cells and tissues.
  • This tool has substantial potential for advancing the understanding of nuclear ATP-related biological processes, particularly in cancer.