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Updated: Aug 9, 2025

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species
Published on: May 29, 2016
The genome sequence of Synechocystis sp. PCC 6803 substrain GT-T and its implications for the evolution of PCC 6803
Satu Koskinen1, Juha Kurkela1, Markéta Linhartová2
1Department of Life Sciences/Molecular Plant Biology, University of Turku, Finland.
Abstract:
Synechocystis sp. PCC 6803 is a model cyanobacterium, glucose-tolerant substrains of which are commonly used as laboratory strains. In recent years, it has become evident that 'wild-type' strains used in different laboratories show some differences in their phenotypes. We report here the chromosome sequence of our Synechocystis sp. PCC 6803 substrain, named substrain GT-T. The chromosome sequence of GT-T was compared to those of two other commonly used laboratory substrains, GT-S and PCC-M. We identified 11 specific mutations in the GT-T substrain, whose physiological consequences are discussed. We also provide an update on evolutionary relationships between different Synechocystis sp. PCC 6803 substrains.
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