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Related Concept Videos

Proteomics01:33

Proteomics

7.6K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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Related Experiment Video

Updated: Aug 9, 2025

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
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Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

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Finding a vocation for validation: taking proteomics beyond association and location.

Marcus J C Long1,2, Jinmin Liu3,2, Yimon Aye3,2

  • 1University of Lausanne (UNIL) Switzerland.

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|February 16, 2023
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Summary
This summary is machine-generated.

Proteomics technologies, including spatial and proximity proteomics, offer powerful insights into cellular communication. Understanding their strengths and weaknesses is crucial for accurate data interpretation and successful applications in life sciences.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Proteomics technologies have evolved significantly since the 1970s.
  • Advanced techniques like chemoproteomics, spatial proteomics, and proximity proteomics enhance the study of cellular communication.
  • These tools are vital for understanding complex biological decision-making processes.

Purpose of the Study:

  • To provide a perspective on the advantages and limitations of modern proteomics profiling technologies.
  • To guide researchers in the rigorous implementation and interpretation of data from proteomics workflows.
  • To equip both expert and novice users with practical knowledge for applying proteomics in life science investigations.

Main Methods:

  • Comparative analysis of commonly deployed modern proteomics profiling technologies.
  • Discussion based on authors' experience applying diverse proteomics workflows in complex living models.
  • Emphasis on orthogonal functional validations for data interpretation.

Main Results:

  • Identification of key considerations for utilizing various proteomics techniques.
  • Contrasting the strengths and weaknesses of different proteomics approaches.
  • Highlighting the indispensable role of proteomics in modern research.

Conclusions:

  • A thorough understanding of proteomics tool capabilities is essential for robust scientific conclusions.
  • Proteomics is a critical tool in chemical biology, drug discovery, and broader life science research.
  • This perspective aims to foster informed use of proteomics technologies among researchers.