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Screening for Antibody Specificity and Sensitivity with ELISA

Michael J Halat1, Emily Gravens2

  • 1Omega Biologicals, Inc., Bozeman, MT, USA. mhalat@omegabiologicals.com.

Methods in Molecular Biology (Clifton, N.J.)
|February 16, 2023
PubMed

Monoclonal antibodies (MAbs) can be used to detect and quantify protein biomarker antigens (Ag). Systematic screening with an enzyme-linked immunosorbent assay (Butler, J Immunoass, 21(2-3):165-209, 2000) [1] can be used to identify matched Ab-Ag pairs. A method is described for identifying MAbs that recognize cardiac biomarker creatine kinase isoform MB. Cross-reactivity with skeletal muscle biomarker creatine kinase isoform MM and brain biomarker creatine kinase isoform BB is also examined.

Keywords:
AntibodyAntigenCross–reactivitySensitivitySpecificity

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