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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Well-Based Multiplex Food Allergen Colorimetric ELISA.

Robert S Matson1

  • 1QuantiScientifics LLC, Orange, CA, USA. rsmatson@quantiscientifics.com.

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|February 16, 2023
PubMed
Summary

Food allergy poses a global health risk, with over 160 potential allergens identified. Multiplex allergen ELISA offers a way to simultaneously assess patients for multiple food allergies and sensitivities.

Keywords:
AllergensAllergyColorimetricELISAFDAHRPIgEIgGIgG4ImmunoassayIntoleranceMicroarrayMicroplateMultiplexPlasmaProtein extractsStrip-well

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Area of Science:

  • Immunology
  • Allergy Diagnostics

Background:

  • Food allergy is a significant global health concern.
  • Over 160 food groups are known to cause allergic reactions, sensitivities, or intolerances.
  • Enzyme-linked immunosorbent assay (ELISA) is a standard method for diagnosing food allergies.

Purpose of the Study:

  • To describe the development and application of a multiplex allergen ELISA.
  • To enable simultaneous screening for multiple food allergens and intolerances.

Main Methods:

  • Preparation of a multiplex allergen ELISA.
  • Utilizing multiplex immunoassays for simultaneous screening.

Main Results:

  • The multiplex ELISA allows for the assessment of multiple food allergies and sensitivities.
  • Simultaneous screening of patients for various allergens is feasible.

Conclusions:

  • Multiplex allergen ELISA is a valuable tool for assessing food allergy and sensitivity.
  • This method enhances the diagnostic capabilities for complex food allergies.