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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
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Mycotoxin Quantification by Competitive ELISA.

Bryn E Merrill1, Robert S Matson2

  • 1Chapman University, Orange, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 16, 2023
PubMed
Summary
This summary is machine-generated.

This study details a new automated method for preparing corn and wheat samples to detect zearalenone (ZEA), a harmful mycotoxin found in animal feed. The developed procedure enables accurate quantification of ZEA using competitive ELISA, aiding in food safety.

Keywords:
AntibodyAntigenColorimetric spectrophotometryCompetitive ELISAMycotoxinZearalenone

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Area of Science:

  • Agricultural Chemistry
  • Food Safety
  • Analytical Chemistry

Background:

  • Mycotoxins, such as zearalenone (ZEA), contaminate staple crops like corn and wheat.
  • ZEA in animal feed poses reproductive health risks to livestock.
  • Accurate quantification of ZEA is crucial for ensuring animal feed safety.

Purpose of the Study:

  • To describe a procedure for preparing corn and wheat samples for mycotoxin analysis.
  • To develop and present an automated method for sample preparation.
  • To facilitate the quantification of zearalenone (ZEA) in cereal matrices.

Main Methods:

  • Development of an automated sample preparation procedure for corn and wheat.
  • Use of known levels of zearalenone (ZEA) in sample preparation.
  • Analysis of prepared samples using competitive Enzyme-Linked Immunosorbent Assay (ELISA).

Main Results:

  • An automated procedure for preparing corn and wheat samples was successfully developed.
  • The method allows for the quantification of zearalenone (ZEA) in the prepared samples.
  • Competitive ELISA was employed for specific ZEA detection.

Conclusions:

  • The automated sample preparation method is effective for ZEA quantification in corn and wheat.
  • This procedure supports the monitoring of ZEA contamination in animal feed ingredients.
  • Accurate mycotoxin analysis is vital for livestock health and agricultural product safety.