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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
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Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
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An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions
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Interference in ELISA.

Robert S Matson1

  • 1QuantiScientifics LLC, Orange, CA, USA. rsmatson@quantiscientifics.com.

Methods in Molecular Biology (Clifton, N.J.)
|February 16, 2023
PubMed
Summary
This summary is machine-generated.

Enzyme-linked immunosorbent assay (ELISA) accuracy is crucial for clinical decisions. This chapter explores common sample interferences in ELISA tests and provides methods for their identification and removal to ensure reliable results.

Keywords:
ALPAnalytical errorAutoantibodyBiotinDilution linearityEndogenousExogenousFalse negativeFalse positiveHAAAHAMAHRPHeterophileHook effectInterferenceMatrix effectsRecoverySpikeSubstrate inhibition

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Area of Science:

  • Biochemistry
  • Clinical Diagnostics
  • Analytical Chemistry

Background:

  • Enzyme-linked immunosorbent assay (ELISA) is a widely used technique for quantifying biological analytes.
  • Accurate ELISA results are vital for clinical decision-making and patient care.
  • Assay results can be compromised by interfering substances present in biological samples.

Purpose of the Study:

  • To examine the nature of interfering substances in biological samples for ELISA.
  • To discuss methods for identifying and removing these interferences.
  • To validate ELISA assays in the presence of potential interfering substances.

Main Methods:

  • Literature review on ELISA interferences.
  • Analysis of common interfering substances in various biological matrices.
  • Discussion of strategies for interference mitigation and assay validation.

Main Results:

  • Identification of various endogenous and exogenous substances that can interfere with ELISA.
  • Description of techniques for detecting matrix effects in ELISA.
  • Outline of approaches to minimize or eliminate interference, such as sample pretreatment and assay optimization.

Conclusions:

  • Understanding and addressing interferences are critical for maintaining ELISA assay reliability.
  • Implementing appropriate identification and removal strategies enhances the accuracy of clinical diagnostics.
  • Validated ELISA assays provide trustworthy data for effective patient management.