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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Related Experiment Video

Updated: Aug 9, 2025

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

Lateral Flow Microarray-Based ELISA for Cytokines.

Robert S Matson¹

¹QuantiScientifics LLC, Orange, CA, USA. rsmatson@quantiscientifics.com.

Methods in Molecular Biology (Clifton, N.J.)

|February 16, 2023

Summary

This summary is machine-generated.

This study introduces a rapid cytokine detection method using lateral flow microarrays (LFM). This multiplex immunoassay aids in understanding inflammatory responses and conditions like severe COVID-19.

Keywords:

Cytokine ELISA Lateral flow Microarray Multiplex Rapid test

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Last Updated: Aug 9, 2025

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

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Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

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Published on: June 14, 2018

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Published on: October 13, 2017

Area of Science:

Biomedical Engineering
Immunology
Infectious Disease Research

Background:

Cytokines mediate biological responses, including inflammation.
A "cytokine storm" is linked to severe COVID-19.
Multiplex cytokine detection is crucial for understanding these processes.

Purpose of the Study:

To describe the creation and application of multiplex lateral flow immunoassays.
To develop a rapid test for cytokine detection.
To adapt enzyme-linked immunosorbent assay (ELISA) principles for lateral flow microarrays.

Main Methods:

Immobilization of capture anti-cytokine antibodies on a microarray.
Construction of lateral flow microarray (LFM) devices.
Utilizing enzyme-linked immunosorbent assay (ELISA) principles for assay development.

Main Results:

Demonstrated the feasibility of multiplex cytokine detection using LFM.
Developed a rapid immunoassay for simultaneous cytokine analysis.
Established methods for creating and using LFM-based cytokine tests.

Conclusions:

Lateral flow microarray technology offers a viable method for multiplex cytokine detection.
This rapid test can aid in studying inflammatory conditions and diseases like COVID-19.
The described methods provide a foundation for advanced cytokine profiling.