Lateral Flow Microarray-Based ELISA for Cytokines

Cytokines are well known to be involved in numerous biological responses with diverse mechanisms of action, including the inflammatory process. The so-called "cytokine storm" has recently been associated with cases of severe COVID-19 infection.Lateral flow microarray (LFM) devices have been constructed for multiplex detection of cytokines. The LFM-cytokine rapid test involves the immobilization of an array of capture anti-cytokine antibodies. Here, we describe the methods to create and use multiplex lateral flow-based immunoassays based upon the enzyme-linked immunosorbent assay (ELISA).