Well-Based Multiplex Food Allergen Colorimetric ELISA

Food allergy is a well-recognized and significant health hazard around the world. At least 160 food groups have been identified that present allergenic reactions or other sensitivities and intolerance in humans. Enzyme-linked immunosorbent assay (ELISA) is an accepted platform for identifying the nature of the food allergy and its severity. It is now possible to simultaneously screen patients for allergic sensitivity and intolerance to multiple allergens using multiplex immunoassays. This chapter describes the preparation and utility of a multiplex allergen ELISA for the assessment of food allergy and sensitivity in patients.