ELISA-Based Biosensors

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Updated: Aug 9, 2025

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
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Robert S Matson1

  • 1QuantiScientifics LLC, Orange, CA, USA. rsmatson@quantiscientifics.com.

Methods in Molecular Biology (Clifton, N.J.)
|February 16, 2023
PubMed

Keywords:
AmperometricAnalogBio-recognitionBiosensorDigitalDigital ELISA (dELISA)Digital droplet ELISA (ddELISA)ElectrochemicalElectrochemiluminescence (ECL)Enzyme-labeled fluorescence (ELF)ImpedanceLab-on-a-chip (LOC)Limit of detection (LOD)LiposomeLuminolMicrofluidicsMinimal detectable dose (MDD)PotentiometricPre-equilibrium digital ELISA (PEdELISA)Quantum dotsRutheniumTransducerTyramide (TSA)Working electrode

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Enzyme-Linked Immunosorbent Assay01:33

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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