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ELISA-Based Biosensors

Robert S Matson1

  • 1QuantiScientifics LLC, Orange, CA, USA. rsmatson@quantiscientifics.com.

Methods in Molecular Biology (Clifton, N.J.)
|February 16, 2023
PubMed

Abstract:

Enzyme-linked immunosorbent assay (ELISA) is by definition a biosensor. However, not all immuno-biosensors involve the use of enzymes, while other biosensors incorporate ELISA as a key signaling component. In this chapter, we review the role of ELISA in signal amplification, integration with microfluidic systems, digital labeling, and electrochemical detection.

Keywords:
AmperometricAnalogBio-recognitionBiosensorDigitalDigital ELISA (dELISA)Digital droplet ELISA (ddELISA)ElectrochemicalElectrochemiluminescence (ECL)Enzyme-labeled fluorescence (ELF)ImpedanceLab-on-a-chip (LOC)Limit of detection (LOD)LiposomeLuminolMicrofluidicsMinimal detectable dose (MDD)PotentiometricPre-equilibrium digital ELISA (PEdELISA)Quantum dotsRutheniumTransducerTyramide (TSA)Working electrode

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