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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

13.9K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
13.9K

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Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
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ELISA-Based Biosensors.

Robert S Matson1

  • 1QuantiScientifics LLC, Orange, CA, USA. rsmatson@quantiscientifics.com.

Methods in Molecular Biology (Clifton, N.J.)
|February 16, 2023
PubMed
Summary
This summary is machine-generated.

Enzyme-linked immunosorbent assay (ELISA) is a biosensor that enhances signal detection. This review explores ELISA

Keywords:
AmperometricAnalogBio-recognitionBiosensorDigitalDigital ELISA (dELISA)Digital droplet ELISA (ddELISA)ElectrochemicalElectrochemiluminescence (ECL)Enzyme-labeled fluorescence (ELF)ImpedanceLab-on-a-chip (LOC)Limit of detection (LOD)LiposomeLuminolMicrofluidicsMinimal detectable dose (MDD)PotentiometricPre-equilibrium digital ELISA (PEdELISA)Quantum dotsRutheniumTransducerTyramide (TSA)Working electrode

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Area of Science:

  • Biotechnology
  • Biosensor technology
  • Immunological assays

Background:

  • Enzyme-linked immunosorbent assay (ELISA) is fundamentally a biosensor.
  • Not all immuno-biosensors utilize enzymes; some integrate ELISA for signaling.
  • Biosensors offer versatile detection platforms.

Approach:

  • Review of ELISA's role in biosensor signal amplification.
  • Integration of ELISA with microfluidic systems.
  • Exploration of digital labeling and electrochemical detection methods in biosensors.

Key Points:

  • ELISA serves as a critical signaling component in various biosensor designs.
  • Microfluidic integration enhances ELISA-based biosensor efficiency.
  • Digital labeling and electrochemical detection offer advanced signal processing for biosensors.

Conclusions:

  • ELISA is a versatile tool in biosensor development, extending beyond traditional enzyme-based applications.
  • The integration of ELISA with advanced technologies like microfluidics and electrochemical detection broadens biosensor capabilities.
  • Future biosensor research can leverage ELISA for improved sensitivity and multiplexing.