Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Brain ascorbate depletion as a response to stress.

B W Tucker1, B M Tolbert, J E Halver

  • 1College of Ocean and Fisheries Science, Univ. of Wash., Seattle 98195.

International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift Fur Vitamin- Und Ernahrungsforschung. Journal International De Vitaminologie Et De Nutrition
|January 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Minimally Invasive Approach to Ureteral Stricture in Transplant Kidney by Periodic Retrograde Ureteral Stent Placement and Exchange.

Transplantation proceedings·2018
Same author

Flexible Ureterorenoscopy and Laser Lithotripsy for the Treatment of Allograft Kidney Lithiasis.

Transplantation proceedings·2015
Same author

Utilization of ascorbate-2-sulfate in fish.

Fish physiology and biochemistry·2013
Same author

The kinetics of the reaction of methanol with 3,3'-dicarbazylphenylmethyl ion in alkaline solution.

Journal of the American Chemical Society·2010
Same author

Oleic acid content is responsible for the reduction in blood pressure induced by olive oil.

Proceedings of the National Academy of Sciences of the United States of America·2008
Same author

Introduction: historiographic issues.

Journal of the history of biology·2001

Rainbow trout brain ascorbate uptake is slow and turnover is long. Stress from metabolism chambers significantly depleted brain ascorbate, suggesting a separate brain pool.

Area of Science:

  • Biochemistry
  • Animal Physiology
  • Nutritional Science

Background:

  • Ascorbate (vitamin C) is essential for many physiological processes in fish.
  • Rainbow trout often experience ascorbate depletion, impacting their health.
  • Understanding ascorbate metabolism is crucial for aquaculture and fish welfare.

Purpose of the Study:

  • To investigate the kinetics of ascorbic acid uptake and turnover in rainbow trout.
  • To determine the impact of physiological stress on brain ascorbate levels.
  • To propose a model for brain ascorbate compartmentalization.

Main Methods:

  • Ascorbate-depleted rainbow trout were administered a single dose of radiolabeled L-1-14C-ascorbic acid.
  • Fish were housed in metabolism chambers for four consecutive five-day periods.

Related Experiment Videos

  • Tissue samples were analyzed for 14C, ascorbate, and ascorbate-2-sulfate concentrations.

    • Main Results:

    • Brain ascorbate exhibited a prolonged turnover time and a slow uptake rate.
    • A highly significant loss of brain ascorbate (p < 0.001) was observed in fish subjected to metabolism chambers compared to controls.
    • These findings suggest a distinct brain ascorbate pool separate from the general body pool.

    Conclusions:

    • A compartmentalized brain ascorbate pool that does not readily exchange with the systemic circulation is proposed.
    • Metabolism chamber conditions induce significant stress, leading to rapid depletion of this brain ascorbate pool.
    • Further research into the mechanisms and kinetic modeling of this brain ascorbate pool is warranted.