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Screening for Lactic Acid Bacterial Strains as Probiotics Exhibiting Anti-inflammatory and Antioxidative
Min Jae Shin1, Chul Sang Lee2,3, Sae Hun Kim4,5
1College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.
Probiotics and Antimicrobial Proteins
|February 22, 2023
Summary
Limosilactobacillus fermentum MF10 demonstrates potent probiotic capabilities, effectively reducing inflammation and oxidative stress. This lactic acid bacterium shows promise for modulating the gut-skin axis by influencing key biomarkers.
Area of Science:
- Microbiology and Immunology
- Gastroenterology
- Dermatology
Background:
- Lactic acid bacteria (LAB) are recognized for their probiotic potential.
- Investigating LAB's functional properties is crucial for understanding their health benefits.
- Inflammation and oxidative stress models are essential for evaluating probiotic efficacy.
Purpose of the Study:
- To screen and identify LAB strains with significant antioxidant and anti-inflammatory properties.
- To evaluate the survival and functional capabilities of selected LAB in simulated intestinal conditions.
- To assess the impact of promising LAB strains on inflammatory biomarkers, oxidative stress, and mitochondrial activity.
Main Methods:
- Screening of 60 LAB strains for radical scavenging activity.
- Evaluation of LAB survival under low pH and bile salt conditions.
- In vitro assessment of antioxidant, anti-inflammatory, and mitochondrial effects using cell models.
Main Results:
- Three LAB strains (Levilactobacillus brevis D70, Lactiplantibacillus pentosus S16, Limosilactobacillus fermentum MF10) exhibited antioxidant capacity and intestinal survival.
- Limosilactobacillus fermentum MF10 significantly downregulated inflammatory biomarkers (IL-8, IFN-γ) induced by LPS.
- Limosilactobacillus fermentum MF10 upregulated antioxidative mediator GPX4 and regulated mitochondrial membrane potential (MMP).
Conclusions:
- Limosilactobacillus fermentum MF10 possesses superior probiotic characteristics.
- This strain effectively impacts the immune-related gut-skin axis.
- Limosilactobacillus fermentum MF10 demonstrates potential for therapeutic applications in inflammatory and oxidative stress-related conditions.
