Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

7.7K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
7.7K
DNA Isolation01:34

DNA Isolation

193.4K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
193.4K
Non-nuclear Inheritance01:29

Non-nuclear Inheritance

21.6K
Most DNA resides in the nucleus of a cell. However, some organelles in the cell cytoplasm⁠—such as chloroplasts and mitochondria⁠—also have their own DNA. These organelles replicate their DNA independently of the nuclear DNA of the cell in which they reside. Non-nuclear inheritance describes the inheritance of genes from structures other than the nucleus.
21.6K
Export of Mitochondrial and Chloroplast Genes02:19

Export of Mitochondrial and Chloroplast Genes

3.7K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antimicrobial Resistance: How Can We Overcome the Problem?

Antibiotics (Basel, Switzerland)·2026
Same author

Optimized protocol for direct extraction of SARS-CoV-2 RNA from raw wastewater samples (ANRS 0160).

MethodsX·2025
Same author

R-loop control and mitochondrial genome stability require the 5'-3' exonuclease/flap endonuclease OEX1.

The Plant cell·2025
Same author

BCL11b interacts with RNA and proteins involved in RNA processing and developmental diseases.

Biochimica et biophysica acta. Gene regulatory mechanisms·2024
Same author

Definition of a concentration and RNA extraction protocol for optimal whole genome sequencing of SARS-CoV-2 in wastewater (ANRS0160).

The Science of the total environment·2024
Same author

[Wastewater-based epidemiology: is the devil in the details?]

Virologie (Montrouge, France)·2024

Related Experiment Video

Updated: Aug 9, 2025

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
09:54

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Published on: January 5, 2018

15.4K

Mitochondrial DNA Isolation from Plants.

Frédérique Weber-Lotfi1, Arnaud Fertet1, Rokas Kubilinskas1

  • 1Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, Strasbourg, France.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2023
PubMed
Summary
This summary is machine-generated.

Plant mitochondrial DNA (mtDNA) sequencing is challenging due to low copy numbers and complex genomes. This study presents optimized methods for mitochondrial purification and mtDNA extraction to improve plant mtDNA analysis.

Keywords:
ArabidopsisLettuceMitochondriaNGS sequencingPlantsmtDNAqPCR

More Related Videos

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

13.0K
Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
06:53

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Published on: May 5, 2023

2.9K

Related Experiment Videos

Last Updated: Aug 9, 2025

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
09:54

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Published on: January 5, 2018

15.4K
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

13.0K
Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
06:53

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Published on: May 5, 2023

2.9K

Area of Science:

  • Plant Biology
  • Genomics
  • Molecular Biology

Background:

  • Mitochondrial DNA (mtDNA) sequencing is crucial for understanding eukaryotic biology.
  • Plant mtDNA analysis is hindered by low copy number, sequence conservation issues, and complex genome structure.
  • Large nuclear genomes and polyploid plastid genomes in plants further complicate mtDNA sequencing and assembly.

Purpose of the Study:

  • To develop and compare methods for plant mitochondrial purification and mtDNA extraction.
  • To enhance the enrichment of mtDNA for improved sequencing and assembly.
  • To provide standardized protocols for plant mitochondrial genomics.

Main Methods:

  • Purification of plant mitochondria from various species and tissues.
  • Extraction and purification of mtDNA from purified mitochondria.
  • Assessment of mtDNA enrichment using quantitative PCR (qPCR) and Next-Generation Sequencing (NGS) read mapping.

Main Results:

  • Successful purification of plant mitochondria and subsequent mtDNA extraction.
  • Demonstration of varying mtDNA enrichment levels across different procedures.
  • Validation of qPCR and NGS read mapping for assessing mtDNA enrichment.

Conclusions:

  • Mitochondrial purification is essential for efficient plant mtDNA analysis.
  • The presented methods offer improved mtDNA enrichment for plant genomics research.
  • Standardized protocols are needed to overcome challenges in plant mitochondrial genome sequencing.