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Related Concept Videos

RNA Splicing01:32

RNA Splicing

56.1K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Translation01:31

Translation

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Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.0K
Leaky Scanning02:28

Leaky Scanning

5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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Related Experiment Video

Updated: Jun 9, 2025

Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes
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BCL11b interacts with RNA and proteins involved in RNA processing and developmental diseases.

Haitham Sobhy1, Marco De Rovere1, Amina Ait-Ammar2

  • 1University of Strasbourg, UR 7292, DHPI, IUT Louis Pasteur, Schiltigheim, France.

Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms
|October 25, 2024
PubMed
Summary

BCL11b protein interacts with RNA processing and splicing factors, revealing its role in gene expression regulation. This discovery sheds light on developmental disorders and cancer pathways.

Keywords:
BCL11b associated-proteomeBCL11b-associated RNAomeRNA processingTranscription factor BCL11b

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Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • BCL11b is a known transcription regulator and tumor suppressor crucial for development and disease.
  • Its role in HIV latency, cell cycle, differentiation, and apoptosis is established.
  • However, the specific protein complexes BCL11b associates with for gene regulation remain largely unknown.

Purpose of the Study:

  • To identify and characterize the ribonucleoprotein complexes associated with BCL11b.
  • To elucidate the functional implications of these interactions in gene expression and cellular processes.

Main Methods:

  • Utilized CLIP-seq to identify direct RNA binding sites of BCL11b.
  • Employed quantitative LC-MS/MS mass spectrometry to identify interacting proteins.
  • Integrated systems biology approaches for validation and pathway analysis.

Main Results:

  • Identified interactions between BCL11b and key RNA processing/splicing proteins, including FUS, SMN1, UPF1, and Drosha.
  • Demonstrated that BCL11b binds to RNA transcripts and proteins encoded by the same genes (e.g., FUS, ESWR1).
  • Revealed BCL11b's association with nucleoprotein complexes regulating gene expression, impacting cell cycle, development, and disease pathways.

Conclusions:

  • BCL11b interacts with specific protein and RNA components involved in RNA processing and splicing.
  • These interactions are critical for regulating gene expression, including isoform selection.
  • Findings provide a foundation for understanding BCL11b's role in developmental disorders and cancer.