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Updated: Aug 9, 2025

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Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

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362

CRISPR technology: A versatile tool to model, screen, and reverse drug resistance in cancer

Shiva Shirani-Bidabadi1, Aliye Tabatabaee2, Nazita Tavazohi3

  • 1Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan 8174673461, Iran.

European Journal of Cell Biology
|February 22, 2023
PubMed
Abstract

Insights

Clustered regularly interspaced short palindrome repeats (CRISPR) gene editing advances cancer drug resistance research by identifying resistance genes and creating models. CRISPR shows promise in overcoming chemotherapy resistance but requires further study to address limitations.

Area of Science:

  • Oncology
  • Genetics
  • Biotechnology

Background:

  • Drug resistance significantly challenges cancer chemotherapy efficacy.
  • Understanding resistance mechanisms and developing new therapies are critical.
  • CRISPR gene editing offers a powerful approach to study and target cancer drug resistance.

Approach:

  • This review evaluates original research utilizing CRISPR technology for cancer drug resistance.
  • Focus areas include screening resistance-related genes, generating resistant cell/animal models, and genetic manipulation to overcome resistance.
  • Key findings on targeted genes, study models, and drug classes are reported.

Key Points:

  • CRISPR facilitates the identification of genes contributing to drug resistance.
  • CRISPR enables the creation of sophisticated models for studying resistance mechanisms.
  • CRISPR-mediated genetic manipulation can potentially reverse or sensitize cells to chemotherapy.

Conclusions:

  • CRISPR technology is instrumental in dissecting complex cancer drug resistance mechanisms.
  • While effective, CRISPR/Cas9 faces challenges including off-target effects, immunotoxicity, and delivery efficiency.
  • Further research is essential to optimize CRISPR applications for clinical cancer therapy.
Keywords:
CRISPR technologyCancerCancer therapyDrug resistance

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