Establishing a Culex Colony from Field-Collected Eggs

Culex larvae are well adapted to growing and developing in containers, and therefore collecting and rearing field-collected Culex to adulthood in the laboratory is relatively straightforward. What is substantially more challenging is simulating natural conditions that encourage Culex adults to mate, blood feed, and reproduce in laboratory settings. In our experience, this is the most difficult hurdle to overcome when establishing new laboratory colonies. Here, we detail how to collect Culex eggs from the field and establish a colony in the laboratory. Successfully establishing a new colony of Culex mosquitoes in the laboratory will allow researchers to evaluate physiologically, behaviorally, and ecologically relevant aspects of their biology and better understand and manage these important disease vectors.