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MEDIATOR SUBUNIT 16 negatively regulates rice immunity by modulating PATHOGENESIS RELATED 3 activity.
Peng Zhang1,2, Xiaoding Ma3, Lina Liu1
1Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.
Plant Physiology
|February 23, 2023
Summary
Spotted leaf 38 (spl38) in rice, encoding MEDIATOR SUBUNIT 16 (OsMED16), negatively regulates plant immunity and cell death. This finding offers new insights into rice disease resistance mechanisms.
Area of Science:
- Plant Pathology
- Molecular Genetics
- Plant Immunity
Background:
- Lesion mimic mutants (LMMs) are crucial for studying plant programmed cell death and defense.
- Understanding plant immune responses is vital for crop protection.
Purpose of the Study:
- To identify and characterize a novel rice lesion mimic mutant, spotted leaf 38 (spl38).
- To elucidate the role of OsMED16 in rice innate immunity and programmed cell death.
Main Methods:
- Map-based cloning to identify the gene responsible for the spl38 phenotype.
- Genetic analysis of spl38 mutants, OsMED16-overexpressing, and OsMED16-edited rice lines.
- Biochemical assays to investigate protein interactions and enzyme activities.
Main Results:
- spl38 encodes MEDIATOR SUBUNIT 16 (OsMED16).
- spl38 mutants exhibit enhanced resistance to Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo).
- OsMED16 interacts with OsPR3, repressing its chitinase activity and negatively regulating immunity.
Conclusions:
- OsMED16 negatively regulates programmed cell death and innate immunity in rice.
- OsMED16's function in rice immunity contrasts with its Arabidopsis ortholog, likely due to sequence divergence.
- OsMED16 likely modulates rice immunity through the OsPR3-mediated chitin signaling pathway.
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