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Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Measuring the Structure, Composition, and Change of Underwater Environments with Large-area Imaging

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Community-developed checklists for publishing images and image analyses.

Christopher Schmied^1,2, Michael S Nelson³, Sergiy Avilov⁴

¹Fondazione Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milano, Italy.

|February 24, 2023

Summary

New guidelines standardize microscopy image preparation for publications. These community-developed checklists enhance clarity and reproducibility of scientific image data.

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Area of Science:

Biomedical research
Scientific imaging
Microscopy

Background:

Scientific discoveries are increasingly documented through images, especially in biomedical research.
Rapid advancements in microscopy imaging technology generate vast amounts of data.
Lack of unified guidelines for publishing microscopy images and analysis hinders clarity and interpretation.

Approach:

Developed community-driven checklists for preparing light microscopy images and analysis for publication.
Provided key recommendations for image formatting, annotation, color selection, and data availability.
Included guidance for reporting image analysis workflows to ensure transparency.

Key Points:

Checklists address image formatting, annotation, color, data availability, and analysis reporting.
Recommendations aim to improve clarity and interpretability of microscopy images in publications.
Standardization facilitates better understanding and utilization of published image data.

Conclusions:

Implementing these guidelines will enhance the quality and explanatory power of microscopy data in publications.
Increased clarity and reproducibility of image figures are expected outcomes.
These community standards support robust scientific communication in microscopy research.