Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First nationwide full-genome characterisation of human-derived Andes virus in Chile: a retrospective genomic epidemiology study.

The Lancet. Microbe·2026
Same author

Patterns of <i>KRAS</i>, <i>NRAS</i>, and <i>BRAF</i> mutations in colorectal cancer in Chile: a 3-year real-world evidence [2019-2021]-a brief report.

Journal of gastrointestinal oncology·2026
Same author

Estetrol potentiates progesterone-driven growth restraint while blunting pro-angiogenic activation in murine endometriosis lesions.

Cells & development·2026
Same author

Systematic AI-assisted screening of the cadhesome to map epithelial monolayer mechanics.

Cell communication and signaling : CCS·2026
Same author

Prognostic Factors for Relapse and Mortality Risk in Chilean Patients With Testicular Cancer: A Retrospective Cohort Study.

JCO global oncology·2026
Same author

MicroRNA-597 Suppresses Gastric Cancer Invasion and Progression via <i>RUNX1</i> Targeting, an Effect Attenuated by the Long Non-Coding RNA <i>KCNQ1OT1</i>.

International journal of molecular sciences·2026

Related Experiment Video

Updated: Jul 9, 2026

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
06:36

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

Published on: May 13, 2019

Framework for evaluating in vitro vasculogenic mimicry using structural and metabolic parameters.

Gema Nicolle Santander1, Nicole Babbitt1, Javiera Veas-Torres1

  • 1Department of Physiology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, Chile; Millennium Institute on Immunology and Immunotherapy, Santiago 8331150, Chile.

Cell Reports Methods
|July 7, 2026
PubMed
Summary

Vasculogenic mimicry (VM) is the ability of cancer cells to form vessel-like tubes. This study identifies cells capable of VM and environmental factors influencing its formation, crucial for understanding tumor aggressiveness.

Keywords:
CP: cancer biologyMatrigelcancerglucosemechanobiologystandard assayvasculaturevasculogenic mimicryvessel

More Related Videos

A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells
04:00

A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells

Published on: September 7, 2011

Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro
11:03

Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro

Published on: January 31, 2018

Related Experiment Videos

Last Updated: Jul 9, 2026

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
06:36

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

Published on: May 13, 2019

A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells
04:00

A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells

Published on: September 7, 2011

Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro
11:03

Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro

Published on: January 31, 2018

Area of Science:

  • Oncology
  • Cell Biology
  • Biomedical Engineering

Background:

  • Vasculogenic mimicry (VM) involves non-endothelial cells forming vessel-like structures.
  • VM is linked to cancer aggressiveness and poor patient prognosis.
  • Similar structures can form in non-cancerous cells, necessitating clear definitions.

Purpose of the Study:

  • To systematically screen various cell lines for vasculogenic mimicry (VM) capacity.
  • To define in vitro VM formation using advanced imaging techniques.
  • To identify critical environmental factors influencing VM.

Main Methods:

  • Systematic screening of over 23 human and murine cell lines.
  • Confocal microscopy and 3D reconstruction for in vitro VM definition.
  • Assessment of cell density, glucose, serum, and matrix stiffness effects.

Main Results:

  • Identified specific cancer and non-cancer cell types capable of VM.
  • Defined in vitro VM as lumen-containing tubular structures formed by non-endothelial cells.
  • Confirmed correlation between in vitro VM capacity and mouse xenograft models.
  • Determined cell density, glucose, serum, and matrix stiffness as key environmental factors for VM.

Conclusions:

  • Established a reliable method for screening VM capacity in cell lines.
  • Demonstrated that environmental conditions significantly modulate VM formation.
  • Provided a foundation for further research into VM's role in cancer progression and therapeutic targeting.