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Updated: Aug 9, 2025
Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
Published on: March 7, 2022
Expression Pattern and Molecular Mechanism of Oxidative Stress-Related Genes in Myocardial Ischemia-Reperfusion
Jiahe Wu1,2, Jingyi Luo3, Huanhuan Cai1,2
1Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
Abstract:
(1) Background: The molecular mechanism of oxidative stress-related genes (OSRGs) in myocardial ischemia-reperfusion injury (MIRI) has not been fully elucidated. (2) Methods: Differential expression analysis, enrichment analysis, and PPI analysis were performed on the MIRI-related datasets GSE160516 and GSE61592 to find key pathways and hub genes. OSRGs were obtained from the Molecular Signatures Database (MSigDB). The expression pattern and time changes of them were studied on the basis of their raw expression data. Corresponding online databases were used to predict miRNAs, transcription factors (TFs), and therapeutic drugs targeting common differentially expressed OSRGs. These identified OSRGs were further verified in the external dataset GSE4105 and H9C2 cell hypoxia-reoxygenation (HR) model. (3) Results: A total of 134 DEGs of MIRI were identified which were enriched in the pathways of "immune response", "inflammatory response", "neutrophil chemotaxis", "phagosome", and "platelet activation". Six hub genes and 12 common differentially expressed OSRGs were identified. A total of 168 miRNAs, 41 TFs, and 21 therapeutic drugs were predicted targeting these OSRGs. Lastly, the expression trends of Aif1, Apoe, Arg1, Col1a1, Gpx7, and Hmox1 were confirmed in the external dataset and HR model. (4) Conclusions: Aif1, Apoe, Arg1, Col1a1, Gpx7, and Hmox1 may be involved in the oxidative stress mechanism of MIRI, and the intervention of these genes may be a potential therapeutic strategy.
Insights
Key oxidative stress-related genes (OSRGs) like Aif1 and Hmox1 were identified in myocardial ischemia-reperfusion injury (MIRI). Targeting these genes may offer new therapeutic strategies for MIRI treatment.
Area of Science:
- Cardiovascular Biology
- Molecular Medicine
- Genomics
Background:
- The molecular mechanisms of oxidative stress-related genes (OSRGs) in myocardial ischemia-reperfusion injury (MIRI) remain incompletely understood.
- Investigating OSRGs is crucial for developing effective MIRI treatments.
Purpose of the Study:
- To elucidate the role of OSRGs in MIRI.
- To identify key genes, pathways, and potential therapeutic targets for MIRI.
Main Methods:
- Differential gene expression analysis and pathway enrichment on MIRI datasets (GSE160516, GSE61592).
- Identification and analysis of OSRGs from MSigDB.
- Prediction of targeting miRNAs, transcription factors, and drugs.
- Validation using an external dataset (GSE4105) and a cell model.
Main Results:
- 134 differentially expressed genes (DEGs) in MIRI were identified, enriched in immune and inflammatory response pathways.
- Six hub genes and 12 common differentially expressed OSRGs were pinpointed.
- Six specific OSRGs (Aif1, Apoe, Arg1, Col1a1, Gpx7, Hmox1) were confirmed to be involved in MIRI.
Conclusions:
- Aif1, Apoe, Arg1, Col1a1, Gpx7, and Hmox1 are implicated in the oxidative stress mechanisms of MIRI.
- Targeting these identified OSRGs presents a promising therapeutic avenue for MIRI management.
