Hydrogel Based on Nanoclay and Gelatin Methacrylate Polymeric Matrix as a Potential Osteogenic Application
Danielle B Andrade1, Leticya L S Soares2, Francisca L A Cardoso2
1Interdisciplinary Laboratory for Advanced Materials (LIMAV), Materials Science and Engineering Graduate Program (PPGCM), Technology Center, Federal University of Piauí (UFPI), Teresina 64049-550, PI, Brazil.
Journal of Functional Biomaterials
|February 24, 2023
Summary
This study developed a novel nanocomposite hydrogel using gelatin methacrylate (GelMA) and laponite (Lap) for bone regeneration. The biocompatible hydrogel demonstrated osteogenic potential and injectability, paving the way for bone tissue engineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Nanotechnology
Background:
- Bone tissue engineering requires advanced materials for osteogenesis.
- Nanocomposite hydrogels offer promising properties for bone regeneration.
Purpose of the Study:
- To synthesize and characterize a novel nanocomposite hydrogel for osteogenic induction.
- To evaluate the biocompatibility and osteogenic potential of the hydrogel for bone tissue engineering.
Main Methods:
- Hydrogel synthesis using gelatin methacrylate (GelMA) and laponite (Lap) via UV crosslinking.
- Characterization using Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and rheological analysis.
- Biocompatibility assessment via *Artemia salina* lethality test and MTT assay; osteogenic potential evaluated through cell differentiation assays.
Main Results:
- Successful synthesis of a GelMA-Laponite nanocomposite hydrogel.
- Characterization confirmed material interactions, thermal stability, and injectability.
- The hydrogel exhibited excellent cell viability (>80%) and induced stem cell differentiation into osteogenic cells, with no acute toxicity observed.
Conclusions:
- The developed nanocomposite hydrogel shows significant potential for osteogenic induction.
- This material is suitable for applications in bone tissue engineering and regenerative medicine.
