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Biofunctionalization of Magnetic Nanomaterials
Published on: July 16, 2020
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Selenium Bio-Nanocomposite Based on Alteromonas macleodii Mo169 Exopolysaccharide: Synthesis, Characterization, and
Patrícia Concórdio-Reis1,2, Ana Catarina Macedo3,4, Martim Cardeira3,4
1Associate Laboratory i4HB-Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, 2829-516 Caparica, Portugal.
Bioengineering (Basel, Switzerland)
|February 25, 2023
Summary
Marine bacteria exopolysaccharide (EPS) stabilized selenium nanoparticles (SeNPs) showing antioxidant effects. This bio-nanocomposite demonstrated significant cellular antioxidant activity without toxicity at lower concentrations.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Marine Microbiology
Background:
- Exopolysaccharides (EPS) from marine bacteria offer unique properties for biomaterial development.
- Selenium nanoparticles (SeNPs) are explored for various applications, but their stabilization and biocompatibility require optimization.
- Alteromonas macleodii Mo 169 produces a novel EPS with potential as a stabilizing agent.
Purpose of the Study:
- To utilize the novel exopolysaccharide (EPS) from Alteromonas macleodii Mo 169 as a stabilizer and capping agent for selenium nanoparticles (SeNPs).
- To characterize the synthesized EPS/SeNPs bio-nanocomposite.
- To evaluate the cytotoxicity and in vitro antioxidant activity of the EPS and the EPS/SeNPs bio-nanocomposite.
Main Methods:
- Synthesis of selenium nanoparticles (SeNPs) using EPS from Alteromonas macleodii Mo 169 as a stabilizer and capping agent.
- Characterization of SeNPs, including size and morphology analysis.
- Cytotoxicity assessment on human keratinocyte (HaCaT) and fibroblast (CCD-1079Sk) cell lines.
- In vitro evaluation of antioxidant effects by measuring reactive oxygen species (ROS) levels.
Main Results:
- Well-dispersed, spherical SeNPs with an average size of 32 nm were synthesized.
- The EPS alone exhibited no cytotoxicity up to 1 g L-1.
- The EPS/SeNPs bio-nanocomposite showed cytotoxicity only at high concentrations (0.5 and 1 g L-1).
- Non-cytotoxic concentrations of the EPS/SeNPs bio-nanocomposite significantly reduced ROS levels by up to 33.8% in HaCaT cells, indicating antioxidant activity.
Conclusions:
- The EPS from Alteromonas macleodii Mo 169 is an effective stabilizer and surface coating agent for producing SeNP-based bio-nanocomposites.
- The EPS/SeNPs bio-nanocomposite possesses significant in vitro antioxidant properties.
- This bio-nanocomposite holds promise for applications requiring antioxidant activity with controlled cytotoxicity.
