Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bone and soft tissue reaction to Co(II)/Cr(III) ions stimulation in a murine calvaria model: A pioneering in vivo study.

Acta biomaterialia·2023

Same author

Structural covariances of prefrontal subregions selectively associate with dopamine-related gene coexpression and schizophrenia.

Cerebral cortex (New York, N.Y. : 1991)·2023

Same author

Genome-Wide Association Analysis of Fruit Shape-Related Traits in <i>Areca catechu</i>.

International journal of molecular sciences·2023

Same author

Whole-tumor radiomics analysis of T2-weighted imaging in differentiating neuroblastoma from ganglioneuroblastoma/ganglioneuroma in children: an exploratory study.

Abdominal radiology (New York)·2023

Same author

Inhibitory effects of Formononetin on CoCrMo particle-induced osteoclast activation and bone loss through downregulating NF-κB and MAPK signaling.

Cellular signalling·2023

Same author

Development and validation of a CT-based radiomics signature for identifying high-risk neuroblastomas under the revised Children's Oncology Group classification system.

Pediatric blood & cancer·2023

Search research articles

Related Experiment Video

Updated: Aug 9, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

Generation of Conditional Knockout Alleles for PRUNE-1.

Xiaoli Wu¹, Louise R Simard¹, Hao Ding^1,2

¹Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB R3E 0J9, Canada.

|February 25, 2023

Summary

This summary is machine-generated.

Researchers created conditional mouse models for PRUNE1, a gene crucial for brain development and function. These models help study PRUNE1

Keywords:

CRISPR/Cas9 Cre recombinase PRUNE1 cerebellar Purkinje cells conditional null allele in vitro fertilization neurodegeneration zygote electroporation

More Related Videos

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Published on: April 2, 2016

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

Related Experiment Videos

Last Updated: Aug 9, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Published on: April 2, 2016

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

Area of Science:

Genetics
Developmental Biology
Neuroscience

Background:

PRUNE1, a DHH superfamily protein, is vital for embryogenesis and nervous system development.
Mutations in PRUNE1 are linked to microcephaly, brain malformations, and neurodegeneration.
Understanding PRUNE1's cellular function is key for developmental and neurodegenerative disease research.

Purpose of the Study:

To generate conditional mouse alleles for PRUNE1 to study its function during development and disease.
To investigate the role of PRUNE1 in Purkinje cell development and survival.

Main Methods:

Generation of conditional mouse alleles for Prune1 using loxP sites flanking exon 6.
Crossed conditional alleles with ubiquitous Cre transgenic lines for complete PRUNE1 loss.
Utilized Purkinje cell-specific Cre line (Pcp2-Cre) to assess PRUNE1's role in Purkinje cells.
Described an efficient method for generating conditional mouse alleles via zygote electroporation.

Main Results:

Complete loss of PRUNE1 expression led to embryonic defects mirroring Prune1 null embryos.
Loss of PRUNE1 in Purkinje cells resulted in Purkinje cell loss, similar to human disease phenotypes.
The generated conditional alleles are effective tools for studying PRUNE1's spatiotemporal roles.

Conclusions:

Conditional mouse alleles of Prune1 are valuable tools for developmental and neurodegenerative disease research.
These models facilitate the study of PRUNE1's role in Purkinje cell development and disease pathogenesis.
The study provides a method for efficient generation of conditional mouse alleles.