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Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

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Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
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Induced Pluripotent Stem Cells01:13

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Related Experiment Video

Updated: Aug 9, 2025

Author Spotlight: Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures
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Modeling Human Brain Tumors and the Microenvironment Using Induced Pluripotent Stem Cells.

Zahraa I Khamis1,2,3,4, Drishty B Sarker1, Yu Xue1

  • 1Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306, USA.

Cancers
|February 25, 2023
PubMed
Summary

Human pluripotent stem cells offer advanced methods for modeling brain cancers like glioblastoma and medulloblastoma in vitro. These induced pluripotent stem cell (iPSC)-based models improve pathological studies and accelerate drug development for brain tumors.

Keywords:
brain organoidsdisease modelingdrug screening and developmenthuman brain cancerinduced pluripotent stem cell technologyisogenic cellsthree-dimensional (3D) cell culture modelstumor microenvironment

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Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
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Area of Science:

  • Neuro-oncology
  • Stem Cell Biology
  • Cancer Research

Background:

  • Brain cancers are aggressive CNS malignancies with poor prognoses.
  • Modeling brain cancer is challenging due to the brain's complexity.
  • Human pluripotent stem cell technology offers new avenues for in vitro disease modeling.

Purpose of the Study:

  • To review induced pluripotent stem cell (iPSC)-based approaches for modeling human brain cancer.
  • To discuss the utility of iPSCs in creating brain cell types, organoids, and blood-brain barrier models.
  • To highlight advancements in simulating brain tumor mutations using genome editing.

Main Methods:

  • Utilizing human pluripotent stem cells, including induced pluripotent stem cells (iPSCs).
  • Employing genome editing and genetic engineering to mimic cancer mutations.
  • Developing in vitro models such as brain organoids and patient-derived xenografts.

Main Results:

  • iPSCs provide renewable sources for various brain cell types and complex brain models.
  • Genome editing enables simulation of germline and somatic mutations in brain tumors.
  • iPSC-derived models, including 3D organoids, are effective for studying glioblastoma and medulloblastoma.

Conclusions:

  • iPSC-based strategies significantly enhance brain cancer modeling capabilities.
  • Combining iPSC-derived models with patient-derived xenografts will advance drug discovery.
  • These advanced models hold promise for improving therapeutic development for deadly brain cancers.