Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

21.5K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nuclear Aggregates of Polyamines-DNA Complexes Imply a Genomic Electronic Circuit.

IUBMB life·2025
Same author

Updated therapeutics of asthma.

World journal of pediatrics : WJP·2025
Same author

Neutralizing IL-15 Inhibits Tissue-Damaging Immune Response in Ex Vivo Cultured Untreated Celiac Intestinal Mucosa.

Cells·2025
Same author

Bioinformatics Study on Site-Specific Variations of Eotaxin-3, a Key Chemokine in Eosinophilic Esophagitis (EoE).

Genes·2024
Same author

Tritordeum: Promising Cultivars to Improve Health.

Foods (Basel, Switzerland)·2024
Same author

Association between Urinary AGEs and Circulating miRNAs in Children and Adolescents with Overweight and Obesity from the Italian I.Family Cohort: A Pilot Study.

Journal of clinical medicine·2023

Related Experiment Video

Updated: Aug 9, 2025

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

13.9K

miRNAs: The Road from Bench to Bedside.

Giuseppe Iacomino1

  • 1Institute of Food Sciences, National Research Council, Via Roma, 64, 83100 Avellino, Italy.

Genes
|February 25, 2023
PubMed
Summary

MicroRNAs (miRNAs) are key regulators of gene expression with potential as diagnostic biomarkers and therapeutics. Challenges in stability and delivery remain, but ongoing research and clinical trials show promise for miRNA-based treatments.

Keywords:
RNA-based therapeuticcirculating miRNAmiRNApersonalized medicinetheranostic biomarkerstranslational medicine

More Related Videos

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

10.1K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.7K

Related Experiment Videos

Last Updated: Aug 9, 2025

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
11:00

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

13.9K
Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

10.1K
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

17.7K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression post-transcriptionally.
  • miRNA dysregulation is linked to cellular and tissue dysfunction.
  • Extracellular miRNAs in biological fluids show potential as biomarkers.

Purpose of the Study:

  • To provide a comprehensive overview of current knowledge on miRNAs.
  • To discuss pending issues and new opportunities in miRNA-based disease treatment and diagnostics.
  • To highlight the role of miRNAs in next-generation medicine.

Main Methods:

  • Literature review of current research on miRNA function, dysregulation, and applications.
  • Analysis of challenges in miRNA stability, delivery, and bioavailability.
  • Examination of ongoing clinical trials involving anti-miR and miR-mimic molecules.

Main Results:

  • Extracellular miRNAs are identified as promising biomarkers for various conditions.
  • miRNAs demonstrate therapeutic potential, with anti-miR and miR-mimic molecules in clinical trials.
  • Significant challenges in miRNA stability, delivery, and bioavailability persist.

Conclusions:

  • miRNAs offer innovative therapeutic strategies and early diagnostic tools.
  • Overcoming current challenges is crucial for realizing the full potential of miRNA-based medicine.
  • The field is rapidly advancing with increasing biopharmaceutical industry engagement.